[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 7, Issue 14 (10-2019) ::
PEC 2019, 7(14): 101-116 Back to browse issues page
Study of ectomycorrhizal fungi with beech trees in highland beech forests (Farim, Mazandaran province)
Hamed Aghajani * , Mohammad Hojjati , Mohammad Ali Tajick , Moahammad Reza Pourmajidian , Ali Borhani
, hamed_aghajani_85@yahoo.com
Abstract:   (3272 Views)
In this study, the ectomycorhizal fungi from beech trees in highland beech forests of Farim (Mazandaran province) were identified based on extraction of DNA from roots and sequencing the ITS region of nuclear ribosomal DNA. For this purpose, in the altitude of 1500-2100 meters A.S.L, 30 plot and one plant per each plot were selected randomly and samples were taken from roots in depths of 10 cm and 60 cm away from tree trunk. The DNA was extracted from classified root tips. ITS nrDNA was replicated and sequenced using ITS1F and ITS4B or ITS4 primer pairs. Comparison of the obtained sequences with sequences deposited in the gene NCBI database revealed the presence of 15 species of ectomycorrhizal fungi in the roots of these plants. Among the identified species, 12 species including Russula chloroides, Cortinarius trivialis, Russula brevipes, Russula faginea, Russula integriformis, Cortinarius rigens, Cortinarius alpinus, Cortinarius collinitus, Lactarius hepaticus, Lactarius chrysorrheus, Cortinarius alboaggregatus, and Hebeloma bulbiferum are reported for the first time to Iran mycoflora. The genus Cortinarius showed the highest species diversity in the studied area followed by Russula, Lactarius, and Inocybe. The identification of ectomycorrhizal fungi could help us use their potentials in the regeneration and optimal management programs of forests and plantations.
Keywords: Forest Biology, rDNA-ITS, Symbiosis, Forest conservation, Beech
Full-Text [PDF 291 kb]   (1308 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/03/11 | Accepted: 2018/07/14 | Published: 2019/09/30
References
1. Aghajani, H., Hojjati, S.M., Tajick-Ghanbari, M.A., Pourmajidian, M.R., Borhani, A. 2019A. Molecular Identification of Ectomycorrhizal Fungal Communities Associated with Oriental Beech Trees (Fagus orientalis Lipsky) in Hyrcanian Forest of Iran. Iranian Journal of Science and Technology, Transactions A: Science, 43:25-32.https://doi.org/10.1007/s40995-017-0435-2
2. Aghajani, H., Hojjati, S.M., Tajick-Ghanbari, M.A., Pourmajidian, M.R., Borhani, A. 2019B.The relationship between Ectomycorrhizal fungi and some soil chemical properties in beech stands of Farim, Mazandaran province. Iranian Journal of Forest and Poplar Research, 26(4):459-470. 10.22092/ijfpr.2018.118577
3. Agerer, R. 1987-2012. Colour Atlas of Ectomycorrhizae. München, 302 Einhorn-Verlag. Separate pagination.
4. Agerer, R. 2001. Exploration types of ectomycorrhizae. Mycorrhiza, 11(2): 107-114.
5. Bahram, M., Põlme, S., Kõljalg, U., Zarre, S., Tedersoo, L. 2012. Regional and local patterns of ectomycorrhizal fungal diversity and community structure along an altitudinal gradient in the Hyrcanian forests of northern Iran. New Phytologist, 193(2): 465–473.
6. Bakker, M.R., Garbaye, J.Nys, C. 2000. Effect of liming on the ectomycorrhizal status of oak. Forest Ecology and Management, 126(2):121–131.
7. Bruns, T.D. 1995. Thoughts on the processes that maintain local species diversity of ectomycorrhizal fungi. Plant and Soil, 170(1): 63–73.
8. Buée, M., Vairelles, D., Garbaye, J. 2005. Year-round monitoring of diversity and potential metabolic activity of the ectomycorrhizal community in a beech (Fagus sylvatica) forest subjected to two thinning regimes. Mycorrhiza, 15(4): 235–245.
9. Cui, L., Mu, L.Q. 2016. Ectomycorrhizal communities associated with Tilia amurensis trees in natural versus urban forests of Heilongjiang in northeast China. Journal of Forestry Research, 27(2): 401-406.
10. Courty, P.E., Buée, M. Diedhiou,A.G., Frey-Klett, P., Le Tacon, F. Rineau, F., Turpault, M.P., Uroz, S. Garbaye, J. 2010. The role of ectomycorrhizal communities in forest ecosystem processes: New perspectives and emerging concepts. Soil Biology and Biochemistry, 42(5): 679-698.
11. Dickie, I.A., Richardson, S.J. Wiser, S.K. 2009. Ectomycorrhizal fungal communities and soil chemistry in harvested and unharvested temperate Nothofagus rainforests. Canadian Journal of Forest Research, 39(6): 1069-1079.
12. Fernández, N.V., Marchelli, P., Gherghel, F.,Kost,G., Fontenla, S.B. 2015. Ectomycorrhizal fungal communities in Nothofagus nervosa (Raulí): a comparison between domesticated and naturally established specimens in a native forest of Patagonia, Argentina. Fungal Ecology, 18: 36-47.
13. Gardes, M., Bruns, T.D. 1993. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2): 113–118.
14. Gebhardt, S., Neubert,K.,Wöllecke, J., Münzenberger,B., Hüttl, R.F. 2007. Ectomycorrhiza communities of red oak (Quercus rubra L.) of different age in the Lusatian lignite mining district, East Germany. Mycorrhiza, 17(4):279–290.
15. Grebenc, T., Kraigher, H. 2007. Types of ectomycorrhiza of mature beech and spruce at ozone-fumigated and control forest plots. Environmental Monitoring and Assessment, 128(1-3): 47-59.
16. Horton, B.M., Glen,M., Davidson,N.J., Ratkowsky,D., Close,D.C., Wardlaw, T.J.,Mohammed, C. 2013. Temperate eucalypt forest decline is linked to altered ectomycorrhizal communities mediated by soil chemistry. Forest EcologyandManagement, 302: 329-337.
17. Huang, J., Nara,K., Zong, K.,Wang, J.,Xue, S., Peng, K.,Sheng,ZH., Lian, C. 2014. Ectomycorrhizal fungal communities associated with Masson pine (Pinus massoniana) and white oak (Quercus fabri) in a manganese mining region in Hunan Province, China. Fungal Ecology, 9: 1-10.
18. Hibbett, D. 2016. The invisible dimension of fungal diversity. Science, 351(6278): 1150-1151.
19. Hoffland, E., Kuyper, T.W., Wallander, H., Plassard, C., Gorbushina, A.A., Haselwandter, K., Holmström, S., Landeweert, R., Lundström, U.S., Rosling, A., Sen, R., Smits, M.M., van Hees, P.A.W., van Breemen, N. 2004. The role of fungi in weathering. FrontiersinEcologyandthe Environment, 2(5): 258-264.
20. Ishida, T.A., Nara,K., Hogetsu, T. 2007. Host effects on ectomycorrhizal fungal communities: insight from eight host species in mixed conifer-broadleaf forests. NewPhytologist, 174(2): 430-440.
21. Kennedy, P. 2010. Ectomycorrhizal fungi and interspecific competition: species interactions, community structure, coexistence mechanisms, and future research directions. NewPhytologist, 187(4): 895–910.
22. Lapeyrie, F., Ranger,J., Vairelles, D. 1991. Phosphate solubilizing ability of ectomycorrhizal fungi in vitro. Canadian Journal of Botany, 69(2): 342-346.
23. Lang, C., Seven,J., Polle, A. 2011. Host preferences and differential contributions of deciduous tree species shape mycorrhizal species richness in a mixed central European forest. Mycorrhiza, 21(4): 297-308
24. Orlovich, D.A., Cairney, J.W.G. 2004. Ectomycorrhizal fungi in New Zealand: current perspectives and future directions. New Zealand Journal of Botany,42(5):712–738.
25. Meharg, A.A., Cairney, J.W.G. 2000. Co-evolution of mycorrhizal symbionts and their hosts to metal-contaminated environments. Advances in Ecological Research, 30:69-112.
26. Montoya, L., Haug, I.,Bandala, V.M. 2010. Two Lactarius species associated with a relict Fagus grandifolia var. mexicana population in a Mexican montane cloud forest. Mycologia, 102(1):153–162.
27. Morris, M.H., Pérez-Pérez, M.A.,Smith, M.E.,Bledsoe, C.S. 2009. Influence of host species on ectomycorrhizal communities associated with two co-occurring oaks (Quercus spp.) in a tropical cloud forest. FEMS Microbiology Ecology, 69(2): 274-287.
28. Mueller, G.M., Schmit, J.P., Leacock, P.R., Buyck, B., Cifuentes, J., Desjardin, D.E., Halling, R.E., Hjortstam, K., Iturriaga, T., Larsson, K.H., Lodge, D.J. 2007. Global diversity and distribution of macrofungi. BiodiversityConservation, 16(1): 37-48.
29. Nara, K., H. Nakaya, B. Wu, Z. Zhou,Hogetsu, T. 2003. Underground primary succession of ectomycorrhizal fungi in a volcanic desert on Mount Fuji. New Phytologist, 159(3): 743-756.
30. Nouhra, E., Urcelay, C., Longo,S., Fontenla, S. 2012. Differential hypogeous sporocarp production from Nothofagus dombeyi and N. pumilio forests in southern Argentina. Mycologia, 104(1):45–52.
31. Nouhra, E., Urcelay, C., Longo, S., Tedersoo, L. 2013. Ectomycorrhizal fungal communities associated to Nothofagus species in northern Patagonia. Mycorrhiza, 23(6): 487-496.
32. Pena, R., Offermann, C., Simon, J., Naumann, P.S., Geßler, A., Holst, J., Dannenmann, M., Mayer, H., Kögel-Knabner, I., Rennenberg, H., Polle, A.2010. Girdling affects ectomycorrhizal diversity and reveals functional differences of EM community composition in a mature beech forest (Fagus sylvatica). Applied and Environmental Microbiology, 76(6):1831–1841.
33. Peay, K.G., Bruns,T.D., Kennedy,P.G.,Bergemann,S.E.,Garbelotto, M. 2007. A strong species–area relationship for eukaryotic soil microbes: island size matters for ectomycorrhizal fungi. Ecology Letters, 10(6): 470–480.
34. Peterson, R.L., Massicotte, H.B.,Melville, L.H. 2004. Mycorrhizas: anatomy and cellbiology. NRC Research Press.
35. Sakakibara, S.M., Jones,M.D.,Gillespie, M., Hagerman, S.M.,Forrest, M.E.,Simard, S.W.,Durall, D.M. 2002. A comparison of ectomycorrhiza identification based on morphotyping and PCR-RFLP analysis. Mycological Research, 106(08): 868-878.
36. Schoch, C. L., Seifert,K.A., Huhndorf, S., Robert, V., Spouge,J.L., Levesque, C.A.,Chen,W., Miller, A.N. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences, 109(16): 6241-6246.
37. Smith, J.E., Molina, R., Huso, M.M., Luoma, D.L., McKay, D., Castellano, M.A., Lebel, T. and Valachovic, Y.2002. Species richness, abundance, and composition of hypogeous and epigeous ectomycorrhizal fungal sporocarps in young, rotationage, and old-growth stands of Douglas-fir (Pseudotsuga menziesii) in the Cascade Range of Oregon, U.S.A. Canadian Journal of Botany, 80: 186-204.
38. Straatsma, G., Ayer,F., Egli, F. 2001. Species richness, abundance, and phenology of fungal fruit bodies over 21 years in a Swiss forest plot. Mycological Research, 105: 515-523.
39. Smith, S., Read, D. 2008. Mycorrhizal Symbiosis, 3rd ed. Academic press, New York. 800pp.
40. Teasdale, S.E., Beulke, A.K.,Guy, P.L.,Orlovich, D.A. 2013. Environmental barcoding of the ectomycorrhizal fungal genus Cortinarius. Fungal Diversity, 58(1): 299-310.
41. Tedersoo L, Bahram, M., Toots, M., Diedhiou, A.G., Henkel, T.W., Kjøller, R., Morris, M.H., Nara, K., Nouhra, E., Peay, K.G., Polme, S., Ryberg, M.,Smith, M.E., Kõljalg, U. 2012. Towards global patterns in the diversity and community structure of ectomycorrhizal fungi. Molecular Ecology,21(17):4160–4170
42. Toljander, J.F., Eberhardt,U., Toljander, Y.K.,Paul,L.R.,Taylor, A.F.S. 2006. Species composition of an ectomycorrhizal fungal community along a local nutrient gradient in a boreal forest. New Phytologist, 170(4): 873–884.
43. White, T.J., Bruns, T.D.,Lee, S., Taylor, J. 1990. Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In: Innis, M.A., D.H. Gelfand, J.J. Sninsky, and T.J. White (Eds.), PCR protocols: a guide to methods and applications. Academic Press, New York, USA, pp 315–322.
44. Wang, Q., Cheng, G., Liang-Dong, G. 2011. Ectomycorrhizae associated with Castanopsis fargesii (Fagaceae) in a subtropical forest, China. MycologicalProgress, 10(3): 323-332.
45. Wilkinson, D.M., Dickinson, N.M. 1995. Metal resistance in treesthe role of mycorrhizae. Oikos, 72: 298-300
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Aghajani H, Hojjati M, Tajick M A, pourmajidian M R, Borhani A. Study of ectomycorrhizal fungi with beech trees in highland beech forests (Farim, Mazandaran province). PEC 2019; 7 (14) :101-116
URL: http://pec.gonbad.ac.ir/article-1-444-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 7, Issue 14 (10-2019) Back to browse issues page
مجله حفاظت زیست بوم گیاهان Journal of Plant Ecosystem Conservation
Persian site map - English site map - Created in 0.08 seconds with 37 queries by YEKTAWEB 4645