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:: Volume 7, Issue 15 (2-2020) ::
PEC 2020, 7(15): 217-235 Back to browse issues page
Genetic diversity of Haloxylon salicornicum (Moq.) Bunge ex Boiss in Different habitats using ISSR Markers (Case study: Yazd)
Mahdiyeh Tajamoliyan * , Hamid Sodqaeizadeh2 , Kazem Sabagh2 , Asghar Mosleharani,2 , Mohammad hadi Rad2 , Mohhamad Ali Hakimzadeh2
Yazd University, Yazd University , tajamoliyan@gmail.com
2- Yazd University
Abstract:   (2910 Views)
Haloxylon salicornicum (Moq.) Bunge ex Boiss is a shrub that grows in large parts of the central regions of Iran. Due to the high adaptability of this species to the extreme environmental conditions and its role in sand dunes stabilization, dust control as well as its medicinal functions, recognition of its genetic diversity and the selection of superior genotypes is very important. In this study, a set of 12 Inter- simple sequence repeat (ISSR) markers were used to evaluate genetic variation of 30 genotypes from 3 locations in Yazd province. Between 12 primers, only 4 primers were able to produce 39 reproducible bands among which 32 bands were polymorphic (79.5%). The mean rate of Nei’s gene diversity (h') and Shannon’s diversity index (I) were 0.451 and 0.643, respectively.  Primers ISSR 826 and ISSR7 with 9 and 7 alleles showed the highest and lowest number of loci, respectively. The number of alleles in each individual locus varied within a range of 7-15 with the mean of 9.75. Using cluster analysis based on UPGMA algorithm and Jaccard's similarity coefficient, the isolates with similarity level of 45% were divided into 5 groups. Shanon index, Principal Coordinates Analysis (PCoA), and Analysis of Molecular Variance (AMOVA) revealed a significant difference among isolates selected from three regions. Analysis of Molecular Variance showed that the highest genetic diversity rate (89%) was disturbed between isolates and genetic variation within different isolated was determined as 11%. Based on the results, it can be demonstrated that a high genetic diversity within the populations will exist due to the closely distributed Haloxylon community in these studied regions.
Keywords: Bafgh, Genetic diversity, (Haloxylon salicornicum (Moq.) Bunge ex Boiss, ISSR marker
Full-Text [PDF 685 kb]   (546 Downloads)    
Type of Study: Research | Subject: Special
Received: 2019/01/8 | Accepted: 2019/06/10 | Published: 2020/03/17
References
1. Abou El-Nasr, THS., Sherin, A., Mahfouze, Al –Kordy, M. 2013. Assessing Phenotypic and Molecular Variability in Fennel (Foeniculum vulgare) Variet ies. Australian. Journal o f Basic and Applied Sciences, 7: 715-722.
2. Ajabnoor, M.A., Yahya, M.A., Tariq, M., Jayab, AA. 1984. phytochemical and biological studies on saudi medicinal plants. Antidiabetic activity of Hammada salicornica. Fitotrapia, 55(2): 107-109.
3. Alansi, S., Tarroum, M., Al-Qurainy, F., Khan, S., Nadeem, M. 2016. Use of ISSR markers to assess the genetic diversity in wild medicinal Ziziphus spina-christi (L.) Willd. Collected from different regions of Saudi Arabia. Biotechnology & Biotechnological Equipment, 30(5): 942-947.‏
4. Brown, G., Prorembski, D. 1997. The maintenance of species diversity by minature dunes in a sand depleted Halxylon salicornicum community in Kuwait. Journal of Arid Environment, 73: 461-463.
5. Dougrameji, J., Kaul, R. N. 1973. Sand dune reclamation in Iraq: present status and future prospects. Annals of Arid Zone, 11(3,4): 133–144.
6. Fernández –Fernandez, F., Harvey NG., James, CM. 2006. Isolation and characterization of polymorphic microsatellite markers from European pear (Pyrus communis). Molecular Ecology Notes, 6(4): 1036-1041.
7. Ge, XJ., Sun M. 1999. Reproductive biology and genetic diversity of a crypto viviparous mangrove Aegiceras corniculatum (Myrsinaceae) using allozyme and inter simple sequence repeat (ISSR) analysis. Molecular Ecology, 8: 2061–206.
8. Mehdigholi, K., Sheidai, M., Niknan,V., Attar, F., Noormohammadi, Z. 2013. Population structure and genetic diversity of Prunus scoparia in Iran. Annales Botanici Fennici, 50:327–336.
9. Ramantha Rao, V., Riley, R. 1994. The use of biotechnology for conservation and utilization of plant genetic resources. Plant Genet Resour News, 97: 3-20.
10. Runo, M.S., Muluvi, G.M., Odee, D.W. 2004. Analysis of genetic structure in Melia volkensii (Gurke.) populations using random amplified polymorphic DNA. African Journal of Biotechnology, 3(8): 421-425.‏
11. Sheng, Y., Zheng, W., Pei, K., Ma, K. 2005. Genetic variation within and among populations of a dominant desert tree Haloxylon ammodendron (Amaranthaceae) in China. Annals of Botany, 96(2): 245-252.
12. Sicard, D., Nanni, L., Porfiri, O., Bulfon, D., Papa, R. 2005. Genetic diversity of Phaseolus vulgaris L. P. coccineus L. landraces in central Italy. Plant Breeding, 124(5): 464-472.
13. Smith, W. B., Frye, C. T., Veliz, E., Hiebler, S., Taylor, R. C., Hunter, K. L. 2015. Genetic Variability of Maryland and West Virginia Populations of the Federally Endangered Plant Harperella nodosa (Rose) (Apiaceae). Northeastern Naturalist, 22(1): 106-119.‏
14. Soreni, A., Nazer, I V., Fatahi Moghdam, M.R., Ahadi Dolatsara, E. 2012. Genetic diversity of plant populations of motherwort in Iran with markers RAPD. Journal of Agricultural Biotechnology.
15. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalskiand, J.A., Tingey, S.V. 1990. DNA polymorphism mplified by arbitrary Primers are useful as genetic markers. Nucleic Acid Research, 18: 6531-6535.
16. Yan, S., Zheng, W., Pei, K., Ma, K. 2005. Genetic variation within and among populations of a dominant desert Tree Haloxylon ammodendron (Amaranthaceae) in China. Annal Botany, 96(2): 245–252.
17. Zaman, S. 1997. Effects of rainfall and grazing on vegetation yield and cover of two arid rangelands in Kuwait. Environmental Conservation, 24(4): 344-350.‏
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Tajamoliyan M, sodqaeizadeh H, sabagh K, mosleharani, A, Rad M H, hakimzadeh M A. Genetic diversity of Haloxylon salicornicum (Moq.) Bunge ex Boiss in Different habitats using ISSR Markers (Case study: Yazd). PEC 2020; 7 (15) :217-235
URL: http://pec.gonbad.ac.ir/article-1-542-en.html


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Volume 7, Issue 15 (2-2020) Back to browse issues page
مجله حفاظت زیست بوم گیاهان Journal of Plant Ecosystem Conservation
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