Investigation to variation between and within same populations of Diospyros lotus based on leaf morphological characteristics, three populations were selected along a elevation transect (populations A, B and C with 300, 500 and 700 meters above sea level, respectively) and 10 tree of each sex from each site was selected. Some leaves from any tree in the first- September collected and randomly, five leaves per each of the triple replication of research separated in 2013 and 10 leaf morphological traits were measured. According to the result of variance analysis between the populations, all traits except wet weight, leaf shape and width of lamina at 0.1, was significant at p< 0.01. Comparison mean between populations showed that population C had the highest amount of mean in all investigated traits. According to the analysis of variances within population, difference between genotypes of male and female sex in each location, the most of traits investigated of leaf was significant. Finally the best genotypes in per populations were introduced that can use for seed collection to seedling production.